Dialdehyde starch cross-linked aminated gelatin sponges with excellent hemostatic performance and biocompatibility

• Published in: Carbohydrate polymers Vol. 342; p. 122326
• Main Authors: Zhu, Yu-He, Zhou, Chen-Yu, Peng, Xu, Wang, Wei, Liu, Zhuang, Xie, Rui, Pan, Da-Wei, Ju, Xiao-Jie, Chu, Liang-Yin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.10.2024
• Subjects: Aminated gelatin; Animals; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Cross-Linking Reagents - chemistry; Femoral Artery - drug effects; Freeze-drying porogenesis; Gelatin - chemistry; Gelatin - pharmacology; Gelatin Sponge, Absorbable - chemistry; Gelatin Sponge, Absorbable - pharmacology; Hemorrhage - drug therapy; Hemostasis - drug effects; Hemostatic materials; Hemostatics - chemistry; Hemostatics - pharmacology; Humans; Male; Porosity; Rapid hemostasis; Rats; Rats, Sprague-Dawley; Starch - analogs & derivatives; Starch - chemistry; Starch - pharmacology; Hemostatic materials; Aminated gelatin; Freeze-drying porogenesis; Rapid hemostasis
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2024.122326

Developing a hemostatic material suitable for rapid hemostasis remains a challenge. This study presents a novel aminated gelatin sponge cross-linked with dialdehyde starch, exhibiting excellent biocompatibility and hemostatic ability. This aminated gelatin sponge features hydrophilic surface and rich porous structure with a porosity of up to 80 %. The results show that the aminated gelatin sponges exhibit superior liquid absorption capacity and can absorb up to 30–50 times their own mass of simulated body fluid within 5 min. Compared with the commercial gelatin hemostatic sponge and non-aminated gelatin hemostatic sponge, the aminated gelatin hemostatic sponge can accelerate the hemostatic process through electrostatic interactions, demonstrating superior hemostatic performance in both in vitro and in vivo hemostasis tests. The aminated gelatin sponge can effectively control the hemostatic time within 80 s in the in vivo rat femoral artery injury model, significantly outperforming both commercial and non-aminated gelatin sponges. In addition, the aminated gelatin sponge also exhibits good biocompatibility and certain antibacterial properties. The proposed aminated gelatin sponge has very good application prospects for the management of massive hemorrhage. [Display omitted]