Prevalence of Candida dubliniensis in the BCCM/IHEM Biomedical Fungi/Yeasts culture collection (isolates before 1990)

The BCCM/IHEM Biomedical Fungi/Yeasts collection hosts 1200 Candida albicans strains of the Vanbreuseghem mycotheque isolated between 1951 and 1997. From this collection, 469 freeze-dried C. albicans strains, producing chlamydospores, germ tubes and forming green colonies on CHROMagar, all isolated...

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Bibliographic Details
Published inMedical mycology (Oxford) Vol. 40; no. 4; p. 443
Main Authors Martins-Nishikawa, M, Trilles, L, Symoens, F, Swinne, D, Nolard, N
Format Journal Article
LanguageEnglish
Published England 01.08.2002
Subjects
Candida - classification
Candida - genetics
Candida - metabolism
Cell Culture Techniques
DNA Fingerprinting
DNA Primers
DNA, Fungal - analysis
DNA, Fungal - genetics
Introns - genetics
Polymerase Chain Reaction
Prevalence
Xylose - metabolism
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Summary:The BCCM/IHEM Biomedical Fungi/Yeasts collection hosts 1200 Candida albicans strains of the Vanbreuseghem mycotheque isolated between 1951 and 1997. From this collection, 469 freeze-dried C. albicans strains, producing chlamydospores, germ tubes and forming green colonies on CHROMagar, all isolated before 1990, were screened to identify the Candida dubliniensis isolates. Screening was performed in different steps using the growth at 45 degrees C, the assimilation of xylose, the intracellular beta-glucosidase activity test and C. dubliniensis-specific polymerase chain reaction (PCR) with primers from ACT1 intron sequence. Five isolates (1%) were identified as C. dubliniensis: one isolate was not documented, the others were of oropharyngeal origin of which two (1987 and 1990) were from proven human immunodeficiency virus patients.
ISSN:1369-3786
DOI:10.1080/714031120