Novel nanocomposite materials of silver-exchanged clinoptilolite with pre-concentration of Ag(NH3)2+ in water possess enhanced anticancer action

• Published in: Applied nanoscience Vol. 10; no. 12; pp. 4869 - 4878
• Main Authors: Vasylechko, Volodymyr O., Klyuchivska, Ol’ga Yu, Manko, Nazar O., Gryshchouk, Galyna V., Kalychak, Yaroslav M., Zhmurko, Iryna I., Stoika, Rostyslav S.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2020
• Subjects: Chemistry and Materials Science; Materials Science; Membrane Biology; Nanochemistry; Nanotechnology; Nanotechnology and Microengineering; Original Article; Clinoptilolite; ); Cytotoxicity; Pre-concentration; Human tumor cells; Ag(NH
• ISSN: 2190-5509; 2190-5517
• DOI: 10.1007/s13204-020-01353-7

The optimal conditions of sorption of Ag(NH 3 ) 2 + ions under the dynamic conditions by the Transcarpathian clinoptilolite (Western Ukraine) were: temperature of clinoptilolite thermal pre-treatment—150 °C; sorbent grain diameter—0.20 to 0.31 mm; flow rate of Ag(I) solution with the concentration of 1.0 µg mL −1 through the sorbent—3 mL min −1 ; concentration of NH 3 —0.05 mol L −1 ; pH 10.8. The sorptive capacity value of clinoptilolite under the optimal conditions is 4.1 mg of Ag per 1 g of zeolite. The best desorbent of Ag(I) from clinoptilolite is 3.5 M HNO 3 . This desorbent removes 100% of sorbed Ag(I). The solid-phase extraction procedure with clinoptilolite was used to pre-concentrate trace amounts of silver ions in water-ammonia solutions to be finally determined by the atomic absorption method. The proposed method allows to pre-concentrate trace amounts of Ag(NH 3 ) 2 + from solutions with a high content of Cl − ions and other macro-components of natural waters. The linearity of the proposed method was evaluated in the range of 1.2–400 ng mL −1 with the detection limit 0.4 ng mL −1 . The intact clinoptilolite and its composites with silver were used for the treatment of human tumor cells (HepG2, HCT116, КB3-1 lines) and pseudonormal cells (human HEК293 cells and murine J774.2 cells). Silver doping increased cytotoxicity of the intact clinoptilolite, while thermal (500 °C, 2.5 h) pretreatment of silver-containing clinoptilolite decreased its cytotoxicity. These effects depended on tissue origin of the treated cells. Ag(NH 3 ) 2 + -zeolite was more toxic comparing with zeolite treated with aqueous NH 3 for all studied mammalian cells.