Cleavage of ATP by Mouse Uterine Chromatin after in vivo Administration of Oestradiol

• Published in: Zeitschrift für Naturforschung. B, A journal of chemical sciences Vol. 27; no. 10; pp. 1267 - 1270
• Main Author: Rainer Maurer, H.
• Format: Journal Article
• Language: English
• Published: Verlag der Zeitschrift für Naturforschung 01.10.1972
• Subjects: ATPase; mouse uterine chromatin; oestradiol
• ISSN: 0932-0776; 1865-7117
• DOI: 10.1515/znb-1972-1028

The capacity of mouse uterine chromatin, prepared according to TENG and HAMILTON, to degrade 8- C-ATP to ADP, AMP and adenosine and to cleave γ- P-ATP to inorganic phosphate and other still unknown phosphorylated byproducts has been demonstrated over a wide range of conditions of the template assay. The dependence of the reactions on the time course of incubation, on the chromatin quantity and on the temperature was studied. In vivo administration of oestradiol depresses the apparent ATPase activity of mouse uterine chromatin consistently by 10-35 per cent, depending on chromatin preparation, amount and incubation period. The data are compatible with the suggestion that mouse uterine chromatin may contain low quantities of ATPases which are firmly bound to the chromatin structure and which might interfere with the template assay under average conditions, depending on the chromatin preparation. The findings are discussed in view of the possible role of ATPases in uterine chromatin under oestradiol influence.