Tomato NADPH oxidase SlWfi1 interacts with the effector protein RipBJ of Ralstonia solanacearum to mediate host defence

• Published in: Plant, cell and environment Vol. 47; no. 12; pp. 5007 - 5020
• Main Authors: Su, Guan‐Ming, Chu, Li‐Wen, Chien, Chih‐Cheng, Liao, Pei‐Shan, Chiu, Yu‐Chuan, Chang, Chi‐Hsin, Chu, Tai‐Hsiang, Li, Chien‐Hui, Wu, Chien‐Sheng, Wang, Jaw‐Fen, Cheng, Yi‐Sheng, Chang, Chuan‐Hsin, Cheng, Chiu‐Ping
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.12.2024
• Subjects: Bacteria; Bacterial diseases; bacterial wilt; Cell death; H2O2; Host plants; Hydrogen peroxide; NAD(P)H oxidase; Oxidase; Pathogens; Plant bacterial diseases; Plant layout; Proteins; Ralstonia solanacearum; RBOHs; Reactive oxygen species; Respiratory burst oxidase; ROS; Signal transduction; Strains (organisms); T3SS; Tomatoes; Virulence; RBOHs; T3SS; bacterial wilt; cell death; H2O2; ROS
• ISSN: 0140-7791; 1365-3040; 1365-3040
• DOI: 10.1111/pce.15086

Reactive oxygen species (ROS) play a crucial role in regulating numerous functions in organisms. Among the key regulators of ROS production are NADPH oxidases, primarily referred to as respiratory burst oxidase homologues (RBOHs). However, our understanding of whether and how pathogens directly target RBOHs has been limited. In this study, we revealed that the effector protein RipBJ, originating from the phytopathogenic bacterium Ralstonia solanacearum, was present in low‐ to medium‐virulence strains but absent in high‐virulence strains. Functional genetic assays demonstrated that the expression of ripBJ led to a reduction in bacterial infection. In the plant, RipBJ expression triggered plant cell death and the accumulation of H2O2, while also enhancing host defence against R. solanacearum by modulating multiple defence signalling pathways. Through protein interaction and functional studies, we demonstrated that RipBJ was associated with the plant's plasma membrane and interacted with the tomato RBOH known as SlWfi1, which contributed positively to RipBJ's effects on plants. Importantly, SlWfi1 expression was induced during the early stages following R. solanacearum infection and played a key role in defence against this bacterium. This research uncovers the plant RBOH as an interacting target of a pathogen's effector, providing valuable insights into the mechanisms of plant defence. Summary Statement This study demonstrates that a tomato plasma membrane‐bound NADPH oxidase is a direct interactor of a bacterial effector protein to mediate plant defence, providing important new information on the interplay between this devastating pathogen and its host plant.