Models for purulent septic inflammation of the tibia in rats to assess the effect of bioresorbable materials with antimicrobial drugs

• Published in: Geniĭ ortopedii = Genij ortopedii Vol. 29; no. 2; pp. 190 - 203
• Main Authors: Smolentsev, D.V., Lukina, Yu.S., Bionyshev-Abramov, L.L., Serezhnikova, N.B., Vasiliev, M.G., Senyagin, A.N., Pkhakadze, T.Ya
• Format: Journal Article
• Language: English
• Published: Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics 01.04.2023
• Subjects: bacterial invasion; cfu; golden staphylococcus aureus; inoculation; osteomyelitis; purulent-septic inflammation; sclerosing agent
• ISSN: 1028-4427; 2542-131X
• DOI: 10.18019/1028-4427-2023-29-2-190-203

Introduction A brief review of modeling purulent septic inflammation in rats, including with the help of an active bacterial agent, and methods for diagnosing inflammation are given. The aim of the study was to demonstrate the results of the development of an effective experimental model of purulent septic inflammation of the tibia in rats using minimally invasive methods for diagnosing infection in vivo. Materials and methods Various models of purulent septic inflammation were studied in four groups of small laboratory animals, when using the inoculation of Staphylococcus aureus. Methods for assessing purulent-septic inflammation that are not destructible by the object have been worked out: microbiological, tomographic, morphological. Results The results of the study indicate the possibility of creating experimental purulent-septic inflammation in rats by 14-60 days using S. aureus inoculation, which is a severe, rapidly progressive purulent infection that leads to extensive destruction of the bone with the formation of sequesters. Discussion To guarantee the formation of a purulent-inflammatory process of bone tissue in a shorter period of observation, a quantitatively controlled invasion of an active bacterial agent is necessary. A sclerosing agent and formation of a fistulous tract are not essential in creating inflammation. Conclusion The results of the development of experimental models for the creation of purulent-septic inflammation using minimally invasive in vivo diagnostic methods are demonstrated, which will allow an adequate assessment of the degree of infection before treatment.