Development of Duplex SYBR Green Real-Time PCR for Rapid and Simultaneous Detection of 16 Specific Genes of 16 Major Foodborne Bacteria

• Published in: JAPANESE JOURNAL OF FOOD MICROBIOLOGY Vol. 30; no. 3; pp. 160 - 164
• Main Authors: FUKUSHIMA, Hiroshi, SUGIYAMA, Kanji, HIROI, Midori, MURAKAMI, Masaru, YAGI, Miya, IIDA, Natsuko, KANDA, Takashi
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society of Food Microbiology 30.09.2013
• Subjects: duplex SYBR Green real-time PCR; foodborne bacteria; simultaneous screening
• ISSN: 1340-8267; 1882-5982
• DOI: 10.5803/jsfm.30.160

[Introduction] In foodborne outbreaks, public health administrators must respond promptly and effectively to prevent the spread of pathogens and the recurrence of food poisoning. To do this, it is necessary to identify the causative agents of outbreaks as soon as possible. Traditional culture methods that have been routinely used require several days to identify foodborne bacteria. Traditional culture methods are time-consuming and laborious, since the necessary culture conditions and nutrient media differ from bacteria to bacteria. Thus, simple and reasonable methods for simultaneously detecting various food-poisoning bacterial species are preferable. Recently, specific gene sequences have been identified in various pathogenic bacteria, and reports on PCR methods targeting the gene sequences of foodborne or waterborne pathogens have been extensively studied 4,7,9). In food poisoning tests, these PCR methods are useful for screening prior to culture. For instance, Fukushima et al. developed a method for simultaneously detecting 24 specific genes of foodborne bacteria using a relatively low-cost multiplex real-time PCR system 3).