Fermentation media and production of exotoxin by three varieties of Bacillus thuringiensis

The insecticidal activities of the exotoxin produced by three varieties of Bacillus thuringiensis grown in six fermentation media were determined by testing the supernatants against larvae of the house fly, Musca domestica, and the black cutworm, Agrotis ipsilon. The activities of the exotoxins from...

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Bibliographic Details
Published inJournal of invertebrate pathology Vol. 35; no. 1; pp. 75 - 83
Main Authors Mohd-Salleh, M.B., Beegle, C.C., Lewis, L.C.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 1980
Subjects
Agrotis ipsilon
Bacillus thuringiensis
Bacillus thuringiensis, exotoxins of
Biological control
Musca domestica
Agrotis ipsilon
Bacillus thuringiensis, exotoxins of
Bacillus thuringiensis
Biological control
Musca domestica
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Summary:The insecticidal activities of the exotoxin produced by three varieties of Bacillus thuringiensis grown in six fermentation media were determined by testing the supernatants against larvae of the house fly, Musca domestica, and the black cutworm, Agrotis ipsilon. The activities of the exotoxins from the isolates varied when they were grown in the same medium and also when they were grown in different media. When an isolate of B. thuringiensis var. thuringiensis, and of var. tolworthi were grown in proflo broth, the supernatants produced were more toxic to house fly than to black cutworm larvae, indicating the presence of more than one exotoxin. Autoclaving the supernatants for 15 and 30 min further demonstrated the presence of several exotoxins.
Bibliography:L
L72
ISSN:0022-2011
1096-0805
DOI:10.1016/0022-2011(80)90086-5