Oxidatiue Phosphorylation in Higher Plant. Part 3

• Published in: Nippon Nōgeikagaku Kaishi Vol. 27; no. 7; pp. 433 - 437
• Main Authors: MARUO, Bunji, MATSUHASHI, Michio
• Format: Journal Article
• Language: Japanese
• Published: Japan Society for Bioscience, Biotechnology, and Agrochemistry 1953
• ISSN: 0002-1407; 1883-6844
• DOI: 10.1271/nogeikagaku1924.27.433

In the papers I and II of this series(1)(2) it was presented that in suitable conditions higher plant (Phaseolus Mungo seedling) homogenates esterify inorganic phosphoate coincident with the aerobic oxidation. It has been made clear by following experiments that the phenomenon of oxidative phos-phorylation takes place within subcellular particles (probably mitochondria). The homog-enate of Phaseolus Mango seedlings is centrifuged (1, 000g. for 15 minutes) and the supern-atant is then recentrifuged (10, 000 g for 15 minutes) and the activities of oxidative phospho-rylation in the supernatant and in the precipitant (R1M) are tested (Table 1). Washed particles (R2M) also hold the activity (Table 2). As shown in Table 3 succinate, malate and α-ketoglutarate are effective as substrates. Taking α-kotglutarate as the substrate, ratio of phosphate esterified to oxygen consumed in the R2M system was found to be one, as seen in Table 4.