recombination-based strategy for assembling open-reading frames

A recombination-based strategy was developed for the assemble of valid open-reading frames. Compared with the conventional reverse transcription-polymerase chain reaction (PCR) approach, this strategy eliminates the inaccurate reverse transcription step but uses the efficient yeast recombination sys...

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Bibliographic Details
Published inPlant molecular biology reporter Vol. 23; no. 4; pp. 397 - 403
Main Authors Chen, X, Tao, Y, Liu, G.Z, Zhu, L.H, Song, W.Y
Format Journal Article
LanguageEnglish
Published New York Springer Nature B.V 01.12.2005
Subjects
Exons
Frames
gene expression
genes
genetic recombination
Introns
kinases
molecular sequence data
new methods
open reading frames
Polymerase chain reaction
Recombination
reverse transcriptase polymerase chain reaction
Reverse transcription
Strategy
transcription (genetics)
Yeast
Yeasts
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Summary:A recombination-based strategy was developed for the assemble of valid open-reading frames. Compared with the conventional reverse transcription-polymerase chain reaction (PCR) approach, this strategy eliminates the inaccurate reverse transcription step but uses the efficient yeast recombination system to assemble the PCR-amplified exons of a gene. This strategy is particularly useful for the construction of full-length open-reading frames disrupted by a small number of introns. The number of valid introns in 862 rice kinase-coding genes was analyzed.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02788887