Fundus angiography with fluorescein-labelled peptide fraction from bovine factor VIII: correlation with histologic findings

A peptide fraction (VUEFFE) obtained from bovine factor VIII, with a high affinity for vessel endothelium, was bound to fluorescein and used for angiography in rabbits. Several eyes had undergone laser photocoagulation on the retina. The location of the dye was studied histologically with fluorescen...

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Bibliographic Details
Published inEuropean journal of ophthalmology Vol. 2; no. 3; p. 135
Main Authors Cardillo Piccolino, F, Ghiglione, D, Ceppa, P, Villaggio, B, Carli, F, Allegri, P, Bertagno, R, Zingirian, M
Format Journal Article
LanguageEnglish
Published United States 01.07.1992
Subjects
Amino Acid Sequence
Animals
Factor VIII
Fluorescein
Fluorescein Angiography
Fluoresceins
Fundus Oculi
Laser Coagulation
Microscopy, Fluorescence
Molecular Sequence Data
Oligopeptides
Rabbits
Retinal Diseases - diagnosis
Retinal Vessels - pathology
Retinal Vessels - surgery
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Summary:A peptide fraction (VUEFFE) obtained from bovine factor VIII, with a high affinity for vessel endothelium, was bound to fluorescein and used for angiography in rabbits. Several eyes had undergone laser photocoagulation on the retina. The location of the dye was studied histologically with fluorescence microscopy on the enucleated, freeze-dried eyes. The angiographic and histologic findings were compared with findings obtained using sodium fluorescein. Fluorescein-labelled VUEFFE gave a longer lasting fluorescence of the retinal vessels with histologic evidence of dye deposition on the vessel wall. It also showed marked affinity for the photocoagulated tissues, giving intense and prolonged staining of the laser burns. Possible clinical applications are discussed.
ISSN:1120-6721
DOI:10.1177/112067219200200305