In vitro propagation of bastard balm (Melittis melissophyllum L.)

An efficient method for propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0...

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Bibliographic Details
Published inHerba polonica Vol. 61; no. 3; pp. 67 - 76
Main Authors Bączek, Katarzyna, Szymborska-Sandhu, Izabela, Pawełczak, Anna, Węglarz, Zenon
Format Journal Article
LanguageEnglish
Published De Gruyter Open 01.09.2015
Polish Ministry of Science and Higher Education
Subjects
adaptacja ex vitro
axillary shoots
ex vitro adaptation
IBA
in vitro
MS/B5 medium
NAA
pożywka MS/B5
pędy boczne
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Summary:An efficient method for propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in conditions very well.
ISSN:2449-8343
2449-8343
DOI:10.1515/hepo-2015-0023