Radiolabelled monoclonal antibodies (McAb): an alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model

• Published in: Archives of pharmacal research Vol. 21; no. 5; pp. 496 - 502
• Main Authors: Haider, K H, Stimson, W H
• Format: Journal Article
• Language: English
• Published: Korea (South) 01.10.1998
• Subjects: Animals; Antibodies, Monoclonal - pharmacokinetics; Autoradiography; Brain Death - diagnosis; Chromatography, Gel; Chromatography, Paper; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Heart - diagnostic imaging; Immunoblotting; Iodine Radioisotopes; Myocardium - pathology; Radionuclide Imaging; Radiopharmaceuticals; Swine; Tissue Distribution
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/BF02975364

The present study was carried out to determine the possible use of cTn-I in the cardiac myofibrillar architecture, as a potential target for in vivo radioimmunodetection of cardiac damage in a brain death pig model. Radioiodination of the anti-cTn-I 5F4 McAb was carried out by lactoperoxidase method. The percentage iodine incorporation achieved was 70-75%. The radioiodinated McAbs were purified on Sephadex G-25 column and characterised by Paper chromatography, Phast Gel electrophoresis and electroimmunoblotting. Radioiodinated anti-cTn-I 5F4 McAbs were employed alongside Pyrophosphate (Tc99m-PPi) and Thallium201 chloride (Tl201) in 24 landrace pigs (brain-dead = 18 & sham-operated = 6). The percentage cardiac uptake of the radiolabelled antibody injected dose was significantly higher in the brain dead animals (0.196%) as compared to that of sham-operated animals (0.11%). Specific in vivo localization of radiolabelled McAbs in the infarcted cardiac tissue was confirmed by computer-aided reconstruction of 3-D images of the isolated heart. The preliminary results of the study revealed preferential uptake of radiolabelled antibody at the site of myocyte damage resulting from artificially induced brain death.