Confirmation of rinderpest in experimentally and naturally infected cattle using microtitre techniques

Virulent rinderpest virus was detected by immunoperoxidase staining of microtitre bovine kidney cell cultures within 24 to 48 hours of inoculation with prescapular lymph node and spleen homogenates from experimentally infected steers. Rinderpest virus specific cytopathic effects were evident from 48...

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Bibliographic Details
Published inTropical animal health and production Vol. 23; no. 1; p. 17
Main Authors Wamwayi, H M, Rossiter, P B, Wafula, J S
Format Journal Article
LanguageEnglish
Published United States 01.02.1991
Subjects
Animals
Cattle
Cells, Cultured
Cytopathogenic Effect, Viral
Disease Outbreaks - veterinary
Immunoenzyme Techniques
Kenya - epidemiology
Lymph Nodes - microbiology
Male
Rinderpest - diagnosis
Rinderpest - epidemiology
Rinderpest virus - isolation & purification
Spleen - microbiology
Kenya
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Summary:Virulent rinderpest virus was detected by immunoperoxidase staining of microtitre bovine kidney cell cultures within 24 to 48 hours of inoculation with prescapular lymph node and spleen homogenates from experimentally infected steers. Rinderpest virus specific cytopathic effects were evident from 48 hours in microtitre plates and from 72 hours in rolled tube cultures. Nasal and ocular secretions collected from cattle naturally infected with rinderpest and inoculated into bovine kidney cell cultures did not readily yield cytopathic virus in both tubes and microtitre plates, but immunoperoxidase staining of microtitre cultures on the fourth day of inoculation detected replication of virus in cultures inoculated with ocular and nasal secretions from seven of 17 cattle tested.
ISSN:0049-4747
DOI:10.1007/BF02361264