Sarcoplasmic reticulum and intermediate filament organization in cultured neonatal cardiac muscle cells. Studies with reduced osmium ferrocyanide

Cardiac muscle cells from 3-day-old rat neonates were cultured for periods of 2 to 56 days. In order to facilitate ultrastructural studies on the organization of the sarcoplasmic reticulum, the cells were prepared for transmission electron microscopy according to a regimen including postfixation in...

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Bibliographic Details
Published inCell and tissue research Vol. 228; no. 3; p. 489
Main Authors Moses, R L, Delcarpio, J B
Format Journal Article
LanguageEnglish
Published Germany 1983
Subjects
Animals
Animals, Newborn
Cells, Cultured
Cytoskeleton - ultrastructure
Ferrocyanides
Fixatives
Microscopy, Electron
Myocardium - ultrastructure
Osmium Tetroxide
Rats
Sarcoplasmic Reticulum - ultrastructure
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Summary:Cardiac muscle cells from 3-day-old rat neonates were cultured for periods of 2 to 56 days. In order to facilitate ultrastructural studies on the organization of the sarcoplasmic reticulum, the cells were prepared for transmission electron microscopy according to a regimen including postfixation in reduced osmium ferrocyanide. The nonjunctional sarcoplasmic reticulum (NJSR) was organized as a loose, fenestrated sleeve around the exterior of bundles of myofilaments and was particularly prominent at the level of the Z line. The only recognizable junctional elements of the sarcoplasmic reticulum were in a peripheral location. Reduced osmium ferrocyanide was also useful in distinguishing intermediate (10 nm) filaments, since it understained Z substance, which often obscured these structures. Intermediate filaments were arranged both at the Z line and the intercalated disc, in parallel strands, approximately at right angles to the myofilaments.
ISSN:0302-766X
DOI:10.1007/BF00211470