Affinity purification of penicillin acylase on phenylacetic acid linked to Indion 48-R: Effect of spacer variation

Affinity matrices, obtained by coupling the ligand phenylacetic acid to Indion 48-R (a macroporous cross linked polystyrene anion exchange resin) through varying spacers, were used to purify penicillin acylase (PA). Homogenous enzyme preparations could be obtained in a single step with over 85% reco...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology techniques Vol. 3; no. 3; pp. 145 - 148
Main Authors Karyekar, S. K., Hegde, M. V.
Format Journal Article
LanguageEnglish
Published 01.03.1989
Subjects
Escherichia coli
Indion 48-R
penicillin acylase
phenylacetic acid
Online AccessGet full text

Cover

More Information
Summary:Affinity matrices, obtained by coupling the ligand phenylacetic acid to Indion 48-R (a macroporous cross linked polystyrene anion exchange resin) through varying spacers, were used to purify penicillin acylase (PA). Homogenous enzyme preparations could be obtained in a single step with over 85% recovery. The effect of spacer variations on the adsorption and elution behaviour of PA is discussed.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF01875610