The mechanistic insights of essential oil of Mentha piperita to control Botrytis cinerea and the prospection of lipid nanoparticles to its application

• Published in: Microbiological research Vol. 286; p. 127792
• Main Authors: Fuentes, Juan Mauricio, Jofré, Ignacio, Tortella, Gonzalo, Benavides-Mendoza, Adalberto, Diez, María Cristina, Rubilar, Olga, Fincheira, Paola
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2024
• Subjects: Action mechanisms; Botrytis cinerea; Essential oil of Mentha piperita; Fungal inhibition; Solid lipid nanoparticles; Fungal inhibition; Essential oil of Mentha piperita; Botrytis cinerea; Action mechanisms; Solid lipid nanoparticles
• ISSN: 0944-5013; 1618-0623; 1618-0623
• DOI: 10.1016/j.micres.2024.127792

Botrytis cinerea is the phytopathogenic fungus responsible for the gray mold disease that affects crops worldwide. Essential oils (EOs) have emerged as a sustainable tool to reduce the adverse impact of synthetic fungicides. Nevertheless, the scarce information about the physiological mechanism action and the limitations to applying EOs has restricted its use. This study focused on elucidating the physiological action mechanisms and prospection of lipid nanoparticles to apply EO of Mentha piperita. The results showed that the EO of M. piperita at 500, 700, and 900 μL L−1 inhibited the mycelial growth at 100 %. The inhibition of spore germination of B. cinerea reached 31.43 % at 900 μL L−1. The EO of M. piperita decreased the dry weight and increased pH, electrical conductivity, and cellular material absorbing OD260 nm of cultures of B. cinerea. The fluorescence technique revealed that EO reduced hyphae width, mitochondrial activity, and viability, and increased ROS production. The formulation of EO of M. piperita loaded- solid lipid nanoparticles (SLN) at 500, 700, and 900 μL L−1 had particle size ∼ 200 nm, polydispersity index < 0.2, and stability. Also, the thermogravimetric analysis indicated that the EO of M. piperita-loaded SLN has great thermal stability at 50 °C. EO of M. piperita-loaded SLN reduced the mycelial growth of B. cinerea by 70 %, while SLN formulation (without EO) reached 42 % inhibition. These results supported that EO of M. piperita-loaded SLN is a sustainable tool for reducing the disease produced by B. cinerea. •Essential oil (EO) of Mentha piperita decrease mycelia growth of Botrytis cinerea.•EO of M. piperita promotes the release of cell constituents of B. cinerea.•EO of M. piperitaalters fluidity of cell membrane and mitochondrial activity.•Solid lipid nanoparticles containing EO of M. piperita has antifungal activity.