Conformational properties of the -35 region of the trp promoter in solution: comparison of the wild-type sequence with an AT transversion

• Published in: European biophysics journal Vol. 22; no. 2; pp. 135 - 143
• Main Authors: Lane, A N, Bauer, C J, Frenkiel, T A, Birchall, A J
• Format: Journal Article
• Language: English
• Published: Germany 01.06.1993
• Subjects: Adenine - chemistry; Base Sequence; Magnetic Resonance Spectroscopy - methods; Molecular Sequence Data; Mutation; Promoter Regions, Genetic - genetics; Protein Conformation; Solutions; Temperature; Thermodynamics; Thymidine - chemistry; Tryptophan - chemistry; Tryptophan - genetics
• ISSN: 0175-7571; 1432-1017
• DOI: 10.1007/BF00196917

The majority of the 1H NMR resonances of the protons in a tetradecamer containing the -35 region of the trp promoter d(GCTGTTGACAATTA): d(TAATTGTCAACAGC) and in the TA transversion have been assigned. The conformational properties of the nucleotides have been determined and compared in the two duplexes. Analysis of spin-spin coupling and NOEs shows that all sugar puckers are in the south domain (i.e. near C2' endo) and the glycosidic torsion angles are anti (chi approximately 110 degrees). The NMR data are consistent with the duplex being in the B family of conformations. Significant differences in chemical shifts between the two molecules were observed only for nearest neighbours to the transversion site, suggesting the absence of long range conformational effects. This was confirmed by the similarity of coupling constants and NOEs. Other properties are also not greatly affected at positions more than two base pairs from the mutation site. These results are consistent with the hypothesis that unconstrained oligonucleotides are highly flexible, and can readily accommodate significant perturbations of the local structure, such as a transversion.