16S rRNA Gene Sequence-based Microbial Diversity Analyses of the Geothermal Areas of Cisolok, Kamojang, and Likupang in Indonesia

• Published in: Korean Journal of Microbiology and Biotechnology Vol. 40; no. 3
• Main Authors: Seo, M.J., Korea Food Research Institute, Sungnam, Republic of Korea, Kim, J.N., Yonsei University, Seoul, Republic of Korea, Pyun, Y.R., Yonsei University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 01.09.2012
• Subjects: 16S rRNA gene; INDONESIA; INDONESIE; microbial diversity
• ISSN: 1598-642X
• DOI: 10.4014/kjmb.1205.05001

Microbial diversity analyses were performed in several geothermal areas in Indonesia using a culture-independent approach with 16S rRNA gene sequencing. All areas and the majority of samples were noted as being affiliated with Proteobacteria. In addition, unclassified bacteria with no phylum affiliation were detected at an incidence rate of 20.0-26.5% in every location. The majority groupings in the geothermal hot stream in Cisolok belonged to β-Proteobacteria (27.1%) and Cyanobacteria (11.0%), whereas the majority from the volcanic area in Kamojang was γ-Proteobacteria (51.5%) followed by Aquificales (12.9%). The predominant groups around an underwater thermal vent in the sea at Likupang were γ-Proteobacteria (33.3%) and then Bacteroidetes (27.6%). This detailed microbial community analyses of each area strongly support a possible association with plausible community groups and environmental habitats, such as extremely geothermal or marine habitats. This study has significantly contributed to the expansion of scientific knowledge of the microbial community in Indonesia.