Injectable dextran‐fluorenylmethoxycarbonyl phenylalanine composite hydrogels with improved mechanical properties

• Published in: Polymer international Vol. 70; no. 2; pp. 222 - 229
• Main Authors: Abraham, Jancy N, Joseph, Seena, Trivedi, Rishabh, Karle, Mrunal
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.02.2021; Wiley Subscription Services, Inc
• Subjects: Buffers (chemistry); Crosslinking; Crystallography; Dextran; Dextrans; Fibrillation; Fmoc‐Phe; Hydrogels; Hydrogen bonding; Infrared spectra; injectable gels; Low molecular weights; Mechanical properties; Molecular weight; Phenylalanine; Polysaccharides; rheology; Scanning electron microscopy; Sol-gel processes; Storage modulus
• ISSN: 0959-8103; 1097-0126
• DOI: 10.1002/pi.6118

Low molecular weight hydrogels are recently gaining importance owing to their applications in material sciences and biology. A new class of composite hydrogel was developed using polysaccharides such as dextran and fluorenylmethoxycarbonyl phenylalanine (FmocF) in a phosphate buffer. The molecular weight and concentration of the dextran were varied to obtain rigid but injectable hydrogels without using other crosslinking agents. From the different molecular weights of dextran studied (5k, 40k and 70k), a combination of FmocF (0.6% w/v) and dextran 40k (0.012% w/v) composite gels yielded a maximum value of storage modulus of approximately 1500 Pa, which is 3.5 times higher than the storage modulus of pure FmocF gels. Scanning electron microscopy of FmocF/dextran composite gels revealed highly tangled fibrous structures with dense branches and lower fiber diameter compared to pure FmocF gels. The high‐intensity hydrogen‐bonded N–H peak in the infrared spectra showed enhanced hydrogen bonding in FmocF/dextran composite gels compared to pure FmocF gels. The dextran acts as an impurity in the process of fibrillation, leading to a crystallographic mismatch, and densely packed thin fibers are formed. These gels exhibited gel to sol and sol to gel conversion with temperature or external stress and showed injectable behavior. © 2020 Society of Industrial Chemistry Dextran is incorporated with fluorenylmethoxycarbonyl phenylalanine (FmocF) to form composite hydrogels with enhanced rheological properties showing thixotropic and injectable behavior.