RIA system programming by means of kinetic parameters

Insulin-125I antibody reaction was optimized by physical-chemical parameters. After the activation energies Ea and Ed for association and dissociation, respectively were calculated from the experimental data, the theoretical values of the reaction rate constants ka and kd were determined as well as...

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Bibliographic Details
Published inEuropean journal of nuclear medicine Vol. 4; no. 6; p. 467
Main Authors Silberring, J, Gołda, W
Format Journal Article
LanguageEnglish
Published Germany 01.12.1979
Subjects
Animals
Antigen-Antibody Reactions
In Vitro Techniques
Insulin - immunology
Insulin Antibodies - immunology
Iodine Radioisotopes
Kinetics
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Summary:Insulin-125I antibody reaction was optimized by physical-chemical parameters. After the activation energies Ea and Ed for association and dissociation, respectively were calculated from the experimental data, the theoretical values of the reaction rate constants ka and kd were determined as well as equilibrium constants K. By means of the empirical formulae, the approximate incubation time for the RIA kit and maximal percent of insulin-125I binding to antibody (%B) in relation to temperature were computed. The proposed method may be applied to the new antigen-binder systems preparation (new antibodies, shortening of the incubation time, temperature changes, influence of different ions and kind of buffer).
ISSN:0340-6997
DOI:10.1007/BF00300848