An ultrasensitive green synchronous factorized spectrofluorimetric approach for the simultaneous determination of Favipiravir and Molnupiravir: Application to pharmaceutical, environmental, and biological matrices

• Published in: Luminescence (Chichester, England) Vol. 39; no. 8; pp. e4837 - n/a
• Main Authors: ElSherbiny, Menna S., Eid, Sherif M., Kelani, Khadiga M., Abdel Ghany, Maha F., El‐Olemy, Ahmed, Hasan, Mohamed A., Abbas, Mahmoud M.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2024
• Subjects: Amides - analysis; Amides - blood; Amides - chemistry; Antiviral Agents - analysis; Antiviral Agents - blood; Binary mixtures; Blood plasma; COVID-19; COVID-19 Drug Treatment; Cytidine - analogs & derivatives; Cytidine - analysis; Cytidine - blood; Drinking water; Favipiravir (FVP); Fluorescence; greenness tools; Guidelines; Humans; Hydroxylamines; Limit of Detection; Mercaptopurine - analysis; Mercaptopurine - blood; Molnupiravir (MPV); Pandemics; Pharmaceuticals; Pyrazines - analysis; Pyrazines - blood; Pyrazines - chemistry; SARS-CoV-2; Spectrofluorimetry; Spectrometry, Fluorescence - methods; synchronous spectrofluorimetric; Uridine - analysis; Uridine - blood; Favipiravir (FVP); greenness tools; Molnupiravir (MPV); synchronous spectrofluorimetric
• ISSN: 1522-7235; 1522-7243; 1522-7243
• DOI: 10.1002/bio.4837

During the pandemic, Favipiravir (FVP) and Molnupiravir (MPV) have been widely used for COVID‐19 treatment, leading to their presence in the environment. A green synchronous spectrofluorimetric method was developed to simultaneously detect them in environmental water, human plasma, and binary mixtures. Maximum fluorescence intensity was achieved at pH 8, with MPV exhibiting two peaks at 300 and 430 nm, and FVP showing one peak at 430 nm. A fluorescence subtraction method effectively removed interference, enabling direct determination of MPV at 300 nm and FVP at 430 nm. The method showed linearity within 2–13 ng/mL for FVP and 50–600 ng/mL for MPV, with recoveries of 100.35% and 100.12%, respectively. Limits of detection and quantification were 0.19 and 0.57 ng/mL for FVP and 10.52 and 31.88 ng/mL for MPV. Validation according to ICH and FDA guidelines yielded acceptable results. The method demonstrated good recoveries of FVP and MPV in pharmaceuticals, tap water and Nile water (99.62% ± 0.96% and 99.69% ± 0.64%) as per ICH guidelines and spiked human plasma (94.87% ± 2.111% and 94.79% ± 1.605%) following FDA guidelines, respectively. Its environmental friendliness was assessed using Green Analytical Procedure Index (GAPI) and the Analytical Greenness Metric (AGREE) tools. Favipiravir and Molnupiravir have been extensively used in the past 3 years due to the COVID‐19 pandemic and consequently excreted into the environment, which creates the need to find suitable, sensitive, and cost‐effective method for their analysis.