Properties of calcium carbonate precipitated in the presence of DPPC liposomes modified with the phospholipase A2

• Published in: Journal of thermal analysis and calorimetry Vol. 123; no. 3; pp. 2357 - 2365
• Main Authors: Szcześ, A., Sternik, D.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2016
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Inorganic Chemistry; Measurement Science and Instrumentation; Physical Chemistry; Polymer Sciences; Lipid membranes; Liposomes; Phospholipase A; Calcium carbonate
• ISSN: 1388-6150; 1588-2926
• DOI: 10.1007/s10973-015-4958-5

Small unilamellar dipalmitoylphosphatidylcholine (DPPC) vesicles of average diameter 119 nm were used as a template for calcium carbonate precipitation. The precipitation was carried out from CaCl 2 and Na 2 CO 3 solutions at room temperature 25 °C and physiological temperature 37 °C. Moreover, liposomes modified with the enzyme phospholipase A 2 (PLA 2 ) were also used as an organic matrix. The influence of liposomes on the precipitation of calcium carbonate has been investigated using scanning electron microscopy and X-ray diffraction measurements. The particles surface modification was evidenced using zeta potential measurements and thermogravimetric analysis. The obtained results show that the effect of DPPC vesicles on the CaCO 3 precipitation is concentration dependent. The stability of vaterite increases with the increase in liposomes content in the solutions. At the liposomes concentration equal to 100 mg L −1 a mixture of precipitated vaterite and calcite is observed. The temperature increase weakens this effect. Moreover, the enzyme PLA 2 action has no significant influence on the calcium carbonate precipitation in the presence of DPPC liposomes at both investigated temperatures.