Developmental exposure to arsenic reduces anxiety levels and leads to a depressive-like behavior in female offspring rats: Molecular changes in the prefrontal cortex

• Published in: Neurotoxicology (Park Forest South) Vol. 104; pp. 85 - 94
• Main Authors: Bartos, Mariana, Gallegos, Cristina E., Mónaco, Nina, Lencinas, Ileana, Dominguez, Sergio, Bras, Cristina, del Carmen Esandi, María, Bouzat, Cecilia, Gumilar, Fernanda
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2024
• Subjects: Anxiety; Arsenic; Depressive-like behavior; Neurotoxicity; Oxidative stress; Prefrontal cortex; Depressive-like behavior; Oxidative stress; Neurotoxicity; Anxiety; Prefrontal cortex; Arsenic
• ISSN: 0161-813X; 1872-9711; 1872-9711
• DOI: 10.1016/j.neuro.2024.07.013

Exposure to inorganic arsenic (iAs) detrimentally affects the structure and function of the central nervous system. In-utero and postnatal exposure to iAs has been connected to adverse effects on cognitive development. Therefore, this investigation explores neurobehavioral and neurochemical effects of 0.05 and 0.10 mg/L iAs exposure during gestation and lactation periods on 90-day-old female offspring rats. The assessment of anxiety- and depressive-like behaviors was conducted through the application of an elevated plus maze and a forced swim test. The neurochemical changes were evaluated in the prefrontal cortex (PFC) through the determination of enzyme activities and α1 GABAA subunit expression levels. Our findings revealed a notable impact of iAs exposure on anxiety and the induction of depressive-like behavior in 90-day-old female offspring. Furthermore, the antioxidant status within the PFC exhibited discernible alterations in exposed rats. Notably, the activities of acetylcholinesterase and glutamate pyruvate transaminase demonstrated an increase, while glutamate oxaloacetate transaminase activity displayed a decrease within the PFC due to the iAs treatment. Additionally, a distinct downregulation in the mRNA expression of the α1GABAA receptor was observed in this neuronal region. These findings strongly suggest that iAs exposure during early stages of rat development causes significant modifications in brain oxidative stress markers and perturbs the activity of enzymes associated with cholinergic and glutamatergic systems. In parallel, it elicits a discernible reduction in the level of GABA receptors within the PFC. These molecular alterations may play a role in the diminished anxiety levels and the depressive-like behavior outlined in the current investigation. [Display omitted] •Inorganic arsenic (iAs) reduces anxiety levels and in adult female pups.•iAs leads to a depressive-like behavior in 90-day-old offspring rats.•iAs exposure causes modifications in brain oxidative stress markers.•iAs alters cholinergic and glutamatergic systems in the prefrontal cortex (PFC).•iAs elicits a reduction in the level of GABA receptors within the PFC.