Allogeneic Bone Marrow-Derived Mesenchymal Stromal Cells Promote the Regeneration of Injured Skeletal Muscle without Differentiation into Myofibers
Half-stratum laceration was performed on the tibialis anterior muscle of Sprague-Dawley (SD) rats as a skeletal muscle injury model. Bone marrow-derived mesenchymal stromal cells (BMMSCs), which were derived from enhanced green fluorescent protein (GFP) transgenic SD rats, were transplanted into the...
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Published in | Tissue engineering Vol. 10; no. 7-8; pp. 193 - 1112 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Larchmont
Mary Ann Liebert, Inc
01.07.2004
|
Online Access | Get full text |
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Summary: | Half-stratum laceration was performed on the tibialis anterior muscle of Sprague-Dawley (SD) rats
as a skeletal muscle injury model. Bone marrow-derived mesenchymal stromal cells (BMMSCs),
which were derived from enhanced green fluorescent protein (GFP) transgenic SD rats, were transplanted
into the injured site. Tensile strength produced by nerve stimulation was measured for functional
evaluation before sacrifice. Specimens of the tibialis anterior muscles were stained with hematoxylin
and eosin, and immunohistochemically stained for histological evaluation. Our results showed
that transplanted BMMSCs promoted maturation of myofibers histologically and made the injured
muscle acquire almost normal muscle power functionally by 1 month after transplantation. However,
the results of immunohistochemical staining could not prove that transplanted BMMSCs differentiated
into or fused to skeletal myofibers, although it showed that transplanted BMMSCs
seemed to differentiate into muscle precursor cells. Therefore, our results indicated that BMMSCs
contributed to the regeneration of skeletal muscle by mechanisms other than fusion to myofibers
after differentiation. |
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ISSN: | 1076-3279 1557-8690 |
DOI: | 10.1089/ten.2004.10.1093 |