P 1,P 4-diadenosine 5′ tetraphosphate induces nitric oxide release from bovine aortic endothelial cells

Since the infusion of P 1,P 4-diadenosine 5′ tetraphosphate (Ap4A) into animal models induces vasodilation [1, 2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2-fold greater than the amount o...

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Bibliographic Details
Published inFEBS letters Vol. 427; no. 3; pp. 320 - 324
Main Authors Hilderman, Richard H, Christensen, Eric F
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.05.1998
Subjects
Bovine aortic endothelial cell
Nitric oxide
Receptor
Signal transduction
Signal transduction
Bovine aortic endothelial cell
Receptor
Nitric oxide
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Summary:Since the infusion of P 1,P 4-diadenosine 5′ tetraphosphate (Ap4A) into animal models induces vasodilation [1, 2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2-fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by N G-nitro- l-arginine (L-NNA) and this inhibition was reversed by L-Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)00454-2