In situ detection by monoclonal antibody D-35.1 of cells infected with Marek's disease virus that interact with splenic ellipsoid-associated reticulum cells

• Published in: Avian diseases Vol. 33; no. 4; p. 657
• Main Authors: Jeurissen, S.H.M. (Central Veterinary Institute, Lelystad, The Netherlands), Scholten, R, Hilgers, L.A.T, Pol, J.M.A, Boer, G.F. de
• Format: Journal Article
• Language: English
• Published: United States 01.10.1989
• Subjects: Animals; Antibodies, Monoclonal; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Antigens, Viral - analysis; BAZO; Cell Line; CELLS; CELLULE; CELULAS; Chick Embryo; CHICKENS; ENFERMEDAD DE MAREK; Enzyme-Linked Immunosorbent Assay - veterinary; Fluorescent Antibody Technique; Herpesvirus 2, Gallid - immunology; Herpesvirus 2, Gallid - isolation & purification; HERPETOVIRIDAE; Immunoenzyme Techniques; LINFOCITOS; LYMPHOCYTE; LYMPHOCYTES; MALADIE DE MAREK; MAREKS DISEASE; Mice; Mice, Inbred BALB C; MONOCLONAL ANTIBODIES; non-lymphoid cells; POLLO; POULET; RATE; Specific Pathogen-Free Organisms; SPLEEN; Spleen - cytology; Spleen - microbiology; STAINING
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1591141

Immuno- and enzyme-histochemical staining procedures were used to investigate in vivo the interaction of Marek's disease virus (MDV) with splenic non-lymphoid cells. The newly developed monoclonal antibody D-35.1, which recognizes all three MDV serotypes, was used to study the localization of MDV at various times after intramuscular inoculation of 1-day-old chicks with MDV strain K. The D-35.1-positive cells were detected in the bursa of Fabricius, spleen, thymus, proventriculus, and cecal tonsils, and the number of chickens showing the cells increased between days 4 and 10. From day 21, the skin of the chickens contained D-35.1-positive feather follicles. The D-35.1 monoclonal antibody did not stain any cells in peripheral blood, nerves, kidney, and gonads at any time. In addition, D-35.1-positive cells were not detected in lymphoproliferative lesions in visceral organs and peripheral nerves. Double staining procedures on serial sections using monoclonal antibody CVI-ChNL-68.2, specific for splenic ellipsoid-associated reticulum cells, revealed that the majority of D-35.1-positive cells were situated in the peri-capillary sheath of reticulum cells at day 10. The sheath of cells detected by monoclonal antibody CVI-ChNL-68.2 was disrupted, and they were clustered around D-35.1-positive cells. These results support the hypothesis that ellipsoid-associated reticulum cells are involved in the early pathogenesis of Marek's disease.