A reliable method to quantify sildenafil and its metabolite N-demethylsildenafil by HPLC in plasma of children

The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coeff...

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Published inDrug research (Stuttgart) Vol. 63; no. 9; p. 473
Main Authors Ramírez-Mendiola, B, Flores-Pérez, C, García-Álvarez, R, Juárez-Olguín, H, Flores-Pérez, J, Chávez Pacheco, J L
Format Journal Article
LanguageEnglish
Published Germany 01.09.2013
Subjects
Child
Chromatography, High Pressure Liquid - methods
Humans
Phosphodiesterase 5 Inhibitors - blood
Piperazines - blood
Purines - blood
Sildenafil Citrate
Sulfones - blood
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Summary:The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml-1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil.
ISSN:2194-9379
DOI:10.1055/s-0033-1345183