Curcumin for gastric cancer: Mechanism prediction via network pharmacology, docking, and in vitro experiments

Curcumin originates from the natural herb turmeric, and its antitumor effects have been known about for a long time. However, the mechanism by which curcumin affects gastric cancer (GC) has not been elucidated To elucidate the potential mechanisms of curcumin in the treatment of GC. Network pharmaco...

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Published inWorld journal of gastrointestinal oncology Vol. 16; no. 8; pp. 3635 - 3650
Main Authors Yang, Peng-Hui, Wei, Ya-Nan, Xiao, Bi-Juan, Li, Si-Yi, Li, Xin-Long, Yang, Liang-Jun, Pan, Hua-Feng, Chen, Geng-Xin
Format Journal Article
LanguageEnglish
Published China Baishideng Publishing Group Inc 15.08.2024
Subjects
Basic Study
Survival analysis
Curcumin
Network pharmacology
Gastric cancer
Molecular docking
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Summary:Curcumin originates from the natural herb turmeric, and its antitumor effects have been known about for a long time. However, the mechanism by which curcumin affects gastric cancer (GC) has not been elucidated To elucidate the potential mechanisms of curcumin in the treatment of GC. Network pharmacological approaches were used to perform network analysis of Curcumin. We first analyzed Lipinski's Rule of Five for the use of Curcumin. Curcumin latent targets were predicted using the PharmMapper, SwissTargetPrediction and DrugBank network databases. GC disease targets were mined through the GeneCard, OMIM, DrugBank and TTD network databases. Then, GO enrichment, KEGG enrichment, protein-protein interaction (PPI), and overall survival analyses were performed. The results were further verified through molecular docking, differential expression analysis and cell experiments. We identified a total of 48 curcumin-related genes with 31 overlapping GC-related targets. The intersection targets between curcumin and GC have been enriched in 81 GO biological processes and 22 significant pathways. Following PPI analysis, 6 hub targets were identified, namely, ( ), ( ), ( ), ( ), ( ), and ( ). These factors are correlated with decreased survival rates among patients diagnosed with GC. Molecular docking analysis further substantiated the strong binding interactions between Curcumin and the hub target genes. The experimental findings demonstrated that curcumin not only effectively inhibits the growth of BGC-823 cells but also suppresses their proliferation. mRNA levels of hub targets , , , and in BGC-823 cells were significantly increased in each dose group. Curcumin can play an anti-GC role through a variety of targets, pathways and biological processes.
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Supported by the National Nature Science Foundation of China, No. 81273735 and No. 82174319; the Natural Science Foundation of Guangdong Province, China, No. 2021A1515010961; the Key-Area Research and Development Program of Guangdong Province, China, No. 2020B1111100011; and the China Postdoctoral Science Foundation, China, No. 2023M740859.
Corresponding author: Geng-Xin Chen, MD, Chief Doctor, Centre for Translational Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 111 Dade Road, Guangzhou 510120, Guangdong Province, China. gxchen@gzucm.edu.cn
Author contributions: All authors contributed to the conceptualization and composition of this manuscript; Yang PH, Wei YN and Xiao BJ wrote the paper; Li SY, Li XL and Yang LJ assisted in data collection and analysis; Chen GX and Pan HF contributed to the study design; Chen GX made critical revisions to this paper; All authors have reviewed and approved the final version of the manuscript.
ISSN:1948-5204
1948-5204
DOI:10.4251/wjgo.v16.i8.3635