Cloning and functional characterisation of the mouse P2X7 receptor

We have isolated a 1785‐bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80% and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric r...

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Published inFEBS letters Vol. 439; no. 1-2; pp. 26 - 30
Main Authors Chessell, I.P, Simon, J, Hibell, A.D, Michel, A.D, Barnard, E.A, Humphrey, P.P.A
Format Journal Article
LanguageEnglish
Published England 13.11.1998
Subjects
Amino Acid Sequence
Animals
ATP
Benzoxazoles
Cells, Cultured
Cloning, Molecular
DNA, Complementary - analysis
DNA, Complementary - isolation & purification
Dose-Response Relationship, Drug
Fluorescent Dyes - pharmacokinetics
Humans
Mice
Molecular Sequence Data
Mouse P2X7 receptor
P2Z receptor
Pore
Quinolinium Compounds
Receptors, Purinergic P2 - genetics
Receptors, Purinergic P2 - physiology
Receptors, Purinergic P2X7
Sequence Homology, Amino Acid
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Summary:We have isolated a 1785‐bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80% and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly resembled those of the P2X7 receptor in the native cell line. However, marked phenotypic differences were observed between the mouse receptor, and the other P2X7 receptor orthologues isolated with respect to agonist and antagonist potencies, and the kinetics of formation of the large aqueous pore.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)01332-5