Temperature control technology for PCR

In order to solve the obvious nonlinear problem of temperature and complex structure of PCR instrument during nucleic acid amplification. In this paper, a new nucleic acid amplification device and temperature control algorithm were proposed. In the device, in order to improve the rise and fall rate...

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Bibliographic Details
Published inInternational journal of numerical modelling Vol. 37; no. 5
Main Authors Zhang, Tongxin, Cai, Gaozhe, Zhang, Zhuo, Li, Qian, Cui, Chuanjin
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Inc 01.09.2024
Wiley Subscription Services, Inc
Subjects
Acids
Algorithms
Control algorithms
Control equipment
Control theory
Cooling
Fuzzy control
Fuzzy logic
fuzzy theory
Heat sinks
Kalman filter
Kalman filters
microfluidic
Noise control
Noise measurement
Nonlinear control
Nucleic acids
Particle swarm optimization
PCR
Proportional integral derivative
PSO
Robust control
semiconductor chilling plate
Temperature control
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Summary:In order to solve the obvious nonlinear problem of temperature and complex structure of PCR instrument during nucleic acid amplification. In this paper, a new nucleic acid amplification device and temperature control algorithm were proposed. In the device, in order to improve the rise and fall rate and make the whole reaction device smaller and simpler, this paper uses a microfluidic chip for nucleic acid reaction. At the same time, in the warming and cooling module, the temperature is controlled by the semiconductor chilling plate, the air‐cooled cooling device and the heat sink structure, which greatly improves the speed of nucleic acid amplification. In the algorithm, a hybrid algorithm is designed, using Particle Swarm Optimization (PSO) to optimize PID algorithm parameters, and then based on fuzzy theory, according to the temperature control requirements of nucleic acid amplification, fuzzy rules are analyzed and fuzzy reasoning is carried out, and then combined with PID to achieve rapid response and overshooting control of temperature control. Finally, the measurement noise is filtered by Kalman filter. Finally, COMSOL and MATLAB software are used to simulate and compare, and it is proved that the device has a certain heat dissipation effect in the process of nucleic acid amplification. This algorithm can improve the accuracy and robustness of the control system, improve the response speed, reduce the overshoot, shorten the adjustment time, and restrain the interference.
ISSN:0894-3370
1099-1204
DOI:10.1002/jnm.3280