Development of Cleaved Amplified Polymorphic Sequence Markers for Classifying Ginger ( Zingiber officinale ) Cultivars Using Reference Sequencing

• Published in: Plant breeding and biotechnology Vol. 11; no. 2; pp. 130 - 140
• Main Authors: Kang, Ji-Nam, Lee, Gyeong-Hui, Yu, Jin, Choi, Mi-Hwa, Lee, Simyung
• Format: Journal Article
• Language: English
• Published: 한국육종학회 01.06.2023
• Subjects: 농학
• ISSN: 2287-9358; 2287-9366
• DOI: 10.9787/PBB.2023.11.2.130

Ginger (Zingiber officinale) is grown worldwide in subtropical and tropical regions and primarily used as a spice andmedicinal plant. Despite the economic importance of ginger, research on its molecular aspects is limited. Moreover, although ginger ismainly cultivated through vegetative propagation owing to poor flowering and infertility, few molecular markers have been identifiedto distinguish cultivars. In this research, we developed five Cleaved Amplified Polymorphic Sequence (CAPS) markers that candistinguish between the “Bongdong” ginger (Bg) cultivar, indigenous to South Korea, and the Chinese imported ginger (Cg) cultivarthrough reference sequencing based on the recently reported complete genome information of ginger. Furthermore, the integratedapplication of the five CAPS markers allow us to distinguish between Bg, Cg, and Indonesian ginger. Among them, the ClaI-basedCAPS marker was identified as specific to Bg cultivars. Therefore, TaqMan real-time PCR based on ClaI-based CAPS can be widelyused to distinguish between Bg and Cg cultivars. This study is the first to report the development of genome-based single-nucleotidepolymorphism markers in ginger and therefore provides important information for the breeding and conservation of ginger. KCI Citation Count: 1