Henle Fiber Layer Mapping with Directional Optical Coherence Tomography

• Published in: Retina (Philadelphia, Pa.)
• Main Authors: Kesim, Cem, Bektas, Sevval Nur, Kulali, Zeynep, Yildiz, Erdost, Ersoz, M Giray, Sahin, Afsun, Gunduz-Demir, Cigdem, Hasanreisoglu, Murat
• Format: Journal Article
• Language: English
• Published: United States 01.09.2022
• ISSN: 1539-2864
• DOI: 10.1097/IAE.0000000000003514

To perform a macular volumetric and topographic analysis of Henle Fiber Layer (HFL) from retinal scans acquired by directional optical coherence tomography (D-OCT). 30 healthy eyes of 17 subjects were imaged using the Heidelberg SD-OCT (Spectralis®, Heidelberg Engineering, Heidelberg, Germany) with varied horizontal and vertical pupil entry. Manual segmentation of HFL was performed from retinal sections of horizontally and vertically tilted OCT images acquired within macular 20×20° area. Total HFL volume, mean HFL thickness and HFL coverage area within ETDRS grid were calculated from mapped images. HFL of 30 eyes were imaged, segmented and mapped. The mean total HFL volume was 0.74±0.08 mm3 with 0.16±0.02, 0.18±0.03, 0.17±0.02 and 0.19±0.03 mm3 for superior, temporal, inferior and nasal quadrants, respectively. The mean HFL thickness was 26.5±2.9 µm. Central 1 mm macular zone had the highest mean HFL thickness with 51.0±7.6 µm. The HFL coverage which have thickness equal or above to the mean value had a mean 10.771 ± 0.574 mm2 of surface area. HFL mapping is a promising tool for structural analysis of HFL. Identifying a normative data of HFL morphology will allow further studies to investigate HFL involvement in various ocular and systemic disorders.