Structural Organization of 6B9 Molecule, a Monoclonal Antibody Against Lycopene

• Published in: Monoclonal antibodies in immunodiagnosis and immunotherapy Vol. 36; no. 6; p. 259
• Main Authors: Petyaev, Ivan M, Alekseev, Konstantin P, Tsibezov, Valeriy V, Kostina, Ludmila V, Kozlov, Alexey Y, Kyle, Nigel H, Bashmakov, Yuriy K
• Format: Journal Article
• Language: English
• Published: United States 01.12.2017
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Carotenoids - immunology; Disulfides - chemistry; Immunoglobulin Heavy Chains - chemistry; Immunoglobulin Light Chains - chemistry; Immunoglobulin Variable Region - chemistry; Mice; molecular structure; lycopene; monoclonal antibody; immunotherapy
• ISSN: 2167-9436
• DOI: 10.1089/mab.2017.0041

Full cDNA and corresponding amino acid (AA) sequences of 6B9 monoclonal antibody (mAb) against lycopene was obtained using Step-Out RACE technology. Variable (V) and constant (C) regions were identified. The light chain of 6B9 contained 238 AA IgM with the highest level of identity (0.93) to both the anti-VEGF receptor antibody and anti-collagen type II FAb CIIC1. The heavy chain was composed of 634 AA with a high identity (0.9) to the Ig mu chain C region. Potential posttranslational modification regions in both chains were identified alongside with disulfide bond sites. The obtained information can be used for making chimeric constructs containing 6B9 mAb (or its fragments) and lycopene, a powerful carotenoid with antioxidant as well as antiproliferating properties, which can be implemented in the treatment of an aggressive form of prostate cancer and possibly other malignancies.