Construction of minitype glutamate sensor for in vivo monitoring of l-glutamate in plant

• Published in: Microchemical journal Vol. 188; p. 108505
• Main Authors: Lin, Ye, Yang, Lingfen, Ma, Ying, Ye, Jianshan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023
• Subjects: Cucumber; Electrochemical glutamate sensor; Glutamate oxidase; In vivo; Electrochemical glutamate sensor; In vivo; Glutamate oxidase; Cucumber
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2023.108505

[Display omitted] •A minitype electrochemical sensor for detecting l-glutamate is constructed using GluOx/PMPD/Pt modified GRE.•In vivo monitoring of l-Glu level can be realized in cucumber.•Accurate detection of l-Glu concentration in cucumber juice in vitro.•There are good performances in terms of sensitivity, linear range and anti-interference capability. l-glutamate (l-Glu) is the basic unit for the synthesis of protein, metabolic substances and chlorophyll, and plays an important role in the carbon and nitrogen metabolism in plants. The in vivo monitoring of l-Glu level is of great significance to study the growth and development of plants. Although a variety of methods have been reported for the in vitro determination of l-Glu, there are very limited research focused on the determination of l-Glu in plants. Here we firstly report a simple method to construct a minitype glutamate sensor for in vivo monitoring of l-glutamate in cucumber. The minitype graphite rod electrode (GRE) allows its application for in vivo monitoring, and modification of Pt nanoparticles (PtNPs) and poly (m-phenylenediamine) film (PMPD) significantly improves the sensitivity and anti-interference ability, and immobilization of the glutamate oxidase (GluOx) could effectively catalyze the l-Glu into a detectable H2O2. The resulting l-Glu sensor exhibits a good performance in terms of high sensitivity, good selectivity and excellent stability with a linear range of 2–550 μM and limit of detection (LOD) as low as 0.536 μM. More importantly, the as-developed sensor was successfully applied for the determination of l-Glu in cucumber juice and the in vivo monitoring of l-Glu level in cucumber fruit. We envision that the constructed sensor will show great potentials for studying the growth mechanisms of plants.