Construction of minitype glutamate sensor for in vivo monitoring of l-glutamate in plant
[Display omitted] •A minitype electrochemical sensor for detecting l-glutamate is constructed using GluOx/PMPD/Pt modified GRE.•In vivo monitoring of l-Glu level can be realized in cucumber.•Accurate detection of l-Glu concentration in cucumber juice in vitro.•There are good performances in terms of...
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Published in | Microchemical journal Vol. 188; p. 108505 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.05.2023
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
•A minitype electrochemical sensor for detecting l-glutamate is constructed using GluOx/PMPD/Pt modified GRE.•In vivo monitoring of l-Glu level can be realized in cucumber.•Accurate detection of l-Glu concentration in cucumber juice in vitro.•There are good performances in terms of sensitivity, linear range and anti-interference capability.
l-glutamate (l-Glu) is the basic unit for the synthesis of protein, metabolic substances and chlorophyll, and plays an important role in the carbon and nitrogen metabolism in plants. The in vivo monitoring of l-Glu level is of great significance to study the growth and development of plants. Although a variety of methods have been reported for the in vitro determination of l-Glu, there are very limited research focused on the determination of l-Glu in plants. Here we firstly report a simple method to construct a minitype glutamate sensor for in vivo monitoring of l-glutamate in cucumber. The minitype graphite rod electrode (GRE) allows its application for in vivo monitoring, and modification of Pt nanoparticles (PtNPs) and poly (m-phenylenediamine) film (PMPD) significantly improves the sensitivity and anti-interference ability, and immobilization of the glutamate oxidase (GluOx) could effectively catalyze the l-Glu into a detectable H2O2. The resulting l-Glu sensor exhibits a good performance in terms of high sensitivity, good selectivity and excellent stability with a linear range of 2–550 μM and limit of detection (LOD) as low as 0.536 μM. More importantly, the as-developed sensor was successfully applied for the determination of l-Glu in cucumber juice and the in vivo monitoring of l-Glu level in cucumber fruit. We envision that the constructed sensor will show great potentials for studying the growth mechanisms of plants. |
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ISSN: | 0026-265X 1095-9149 |
DOI: | 10.1016/j.microc.2023.108505 |