Monoclonal antibodies against the 1,4-dihydropyridine receptor associated with voltage-sensitive Ca 2+ channels detect similar polypeptides from a variety of tissues and species

Four monoclonal antibodies have been raised against voltage-sensitive Ca 2+ channel dihydropyridine receptors from rabbit skeletal muscle. When tested by immunoblot assay of denatured transverse tubule membranes in reducing polyacrylamide gels, each recognised a single polypeptide of M r ~ 140000 th...

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Bibliographic Details
Published inFEBS letters Vol. 212; no. 1; pp. 127 - 132
Main Authors Norman, R.I., Burgess, A.J., Allen, E., Harrison, T.M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 09.02.1987
Subjects
Ca 2+ channel
Dihydropyridine
Dihydropyridine receptor
Monoclonal antibody
Dihydropyridine
Monoclonal antibody
Dihydropyridine receptor
Ca 2+ channel
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Summary:Four monoclonal antibodies have been raised against voltage-sensitive Ca 2+ channel dihydropyridine receptors from rabbit skeletal muscle. When tested by immunoblot assay of denatured transverse tubule membranes in reducing polyacrylamide gels, each recognised a single polypeptide of M r ~ 140000 that co-migrated with the large glycoprotein subunit of the purified receptor. In blots of nonreducing gels, a larger protein of M r ~ 170000 was seen and three of the antibodies recognised additional components at M r ~ 310000 and ~ 330000. Crossreactive material of similar molecular mass was also seen in rabbit heart and brain, and in the skeletal muscle of other species.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)81570-3