Biosynthesis of Ag and TiO2 nanoparticles and the evaluation of their antibacterial activities

• Published in: Inorganic chemistry communications Vol. 141; p. 109503
• Main Authors: Jimoh, A.A., Akpeji, B.H, Azeez, S.O., Ayipo, Y.O., Abdulsalam, Z.A., Adebayo, Z.F., Ajao, A.T., Zakariyah, A.T., Elemike, E.E.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2022
• Subjects: AgNPs; Antibacterial; Nanoparticles; TiO2NPs; Nanoparticles; TiO2NPs; AgNPs; Antibacterial
• ISSN: 1387-7003; 1879-0259
• DOI: 10.1016/j.inoche.2022.109503

[Display omitted] •Ag and TiO2 nanoparticles were synthesized using Pseudomonas aeruginosa micro-organism.•The crystalline sizes of the nanoparticles obtained using Image J and Origin software are 7.27 and 6.83 nm for Ag and TiO2NPs respectively.•The antibacterial studies showed great inhibition majorly against the Bacillus spp and the TiO2 NPs was higher in activity more than the AgNPs.•The nanoparticles obtained in this work are more potent than some orthodox antibiotics eg (ciprofloxacin). In this study, Pseudomonas aeruginosa was used for the synthesis of silver and titanium oxide nanoparticles (Ag and TiO2 NPs). The nanoparticles were prepared by mixing the supernatant solution of the cultured organism and the solution of silver nitrate and titanium tetrabutoxide. UV–Vis spectroscopic analysis gave absorption peak at wavelength of 414 nm and 390 nm for Ag and TiO2 NPs respectively. The FTIR spectra gave absorption bands attributed to NH stretch of the peptide linkages, OH stretch of carboxylic acid, amide bend, and CO stretch which may have emanated from the micro-organism. The powdered X-ray diffraction (PXRD) studies revealed the crystalline nature of AgNPs and TiO2 NPs while microscopic studies showed spherical and dispersed morphologies with average particle size of 7.27 nm and 6.83 nm for Ag and TiO2 NPs respectively. Antibacterial analysis of the synthesized nanoparticles showed broad spectrum inhibition against disease causing microorganisms with TiO2NPs exhibiting excellent results more than AgNPs and the control drugs.