Induction and repair of DNA double-strand breaks in hippocampal neurons of miсe of different age after exposure to 60 Со γ-rays in vivo and in vitro
One of the central problems of modern radiobiology is the study of DNA damage induction and repair mechanisms in central nervous system cells, in particular, in hippocampal cells. The study of the regularities of molecular damage formation and repair in the hippocampus cells is of special interest,...
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Published in | EPJ Web of Conferences Vol. 177; p. 6001 |
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Main Authors | , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Les Ulis
EDP Sciences
2018
|
Subjects | |
Online Access | Get full text |
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Summary: | One of the central problems of modern radiobiology is the study of DNA damage induction and repair mechanisms in central nervous system cells, in particular, in hippocampal cells. The study of the regularities of molecular damage formation and repair in the hippocampus cells is of special interest, because these cells, unlike most cells of the central nervous system (CNS), keep proliferative activity, i.e. ability to neurogenesis. Age-related changes in hippocampus play an important role, which could lead to radiosensitivity changes in neurons to the ionizing radiation exposure. Regularities in DNA double-strand breaks (DSB) induction and repair in different aged mice hippocampal cells
in vivo
and
in vitro
under the action of γ-rays
60
Со were studied with DNA comet-assay. The obtained dose dependences of DNA DSB induction are linear both
in vivo
and
in vitro
. It is established that in young animals' cells, the degree of DNA damage is higher than in older animals. It is shown that repair kinetics is basically different for exposure
in vivo
and
in vitro
. |
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ISSN: | 2100-014X 2101-6275 2100-014X |
DOI: | 10.1051/epjconf/201817706001 |