Subclinical peripheral inflammation has systemic effects impacting central nervous system proteome in budgerigars

• Published in: Developmental and comparative immunology Vol. 159; p. 105213
• Main Authors: Voukali, Eleni, Divín, Daniel, Samblas, Mercedes Goméz, Veetil, Nithya Kuttiyarthu, Krajzingrová, Tereza, Těšický, Martin, Li, Tao, Melepat, Balraj, Talacko, Pavel, Vinkler, Michal
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.10.2024
• Subjects: Cerebrospinal fluid; Dextran sulphate sodium; Endotoxin; Parrot; Plasma; Proteomics; Plasma; EPB41; FHL2; PHA; CNS; Cerebrospinal fluid; Endotoxin; BP; LPS; LMNB1; BR; COMP; LC-MS/MS; VTN; FDR; CSF; KEGG; RT-qPCR; PC1; FGB; FGA; Dextran sulphate sodium; IL; DSS; Parrot; FGG; GO; IL18; GSEA; APOA4; CO; ANK1; PCA; IL6; SAA; GIT; IL1B; Proteomics; PL; PLCB1; CRK; FC; Dextran Sulphate Sodium; Cerebrospinal Fluid
• ISSN: 0145-305X; 1879-0089; 1879-0089
• DOI: 10.1016/j.dci.2024.105213

Regulation of neuroimmune interactions varies across avian species. Little is presently known about the interplay between periphery and central nervous system (CNS) in parrots, birds sensitive to neuroinflammation. Here we investigated the systemic and CNS responses to dextran sulphate sodium (DSS)- and lipopolysaccharide (LPS)-induced subclinical acute peripheral inflammation in budgerigar (Melopsittacus undulatus). Three experimental treatment groups differing in DSS and LPS stimulation were compared to controls. Individuals treated with DSS showed significant histological intestinal damage. Through quantitative proteomics we described changes in plasma (PL) and cerebrospinal fluid (CSF) composition. In total, we identified 180 proteins in PL and 978 proteins in CSF, with moderate co-structure between the proteomes. Between treatments we detected differences in immune, coagulation and metabolic pathways. Proteomic variation was associated with the levels of pro-inflammatory cytokine mRNA expression in intestine and brain. Our findings shed light on systemic impacts of peripheral low-grade inflammation in birds. •Parrots represent a novel model to study neuroinflammation.•Subclinical peripheral inflammation in gut or skin has systemic effects in birds.•Low-grade acute peripheral inflammation affects the central nervous system.•SAA was the top inflammatory marker activated in budgerigars by both LPS and DSS.