Unusual aldehyde reductase activity for the production of full-length fatty alcohol by cyanobacterial aldehyde deformylating oxygenase

• Published in: Archives of biochemistry and biophysics Vol. 734; p. 109498
• Main Authors: Treesukkasem, Nidar, Buttranon, Supacha, Intasian, Pattarawan, Jaroensuk, Juthamas, Maenpuen, Somchart, Sucharitakul, Jeerus, Lawan, Narin, Chaiyen, Pimchai, Wongnate, Thanyaporn
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2023
• Subjects: Aldehyde Reductase; Aldehyde-deformylating oxygenase; Aldehydes; Cyanobacteria; Fatty alcohol; Fatty Alcohols; Non-heme diiron enzyme; Oxidoreductase; Oxygen; Oxygenases; Aldehyde-deformylating oxygenase; Fatty alcohol; Oxidoreductase; Non-heme diiron enzyme
• ISSN: 0003-9861; 1096-0384; 1096-0384
• DOI: 10.1016/j.abb.2022.109498

Aldehyde-deformylating oxygenase (ADO) is a non-heme di-iron enzyme that catalyzes the deformylation of aldehydes to generate alkanes/alkenes. In this study, we report for the first time that under anaerobic or limited oxygen conditions, Prochlorococcus marinus (PmADO) can generate full-length fatty alcohols from fatty aldehydes without eliminating a carbon unit. In contrast to ADO's native activity, which requires electrons from the Fd/FNR electron transfer complex, ADO's aldehyde reduction activity requires only NAD(P)H. Our results demonstrated that the yield of alcohol products could be affected by oxygen concentration and the type of aldehyde. Under strictly anaerobic conditions, yields of octanol were up to 31%. Moreover, metal cofactors are not involved in the aldehyde reductase activity of PmADO because the yields of alcohols obtained from apoenzyme and holoenzyme treated with various metals were similar under anaerobic conditions. In addition, PmADO prefers medium-chain aldehydes, specifically octanal (kcat/Km around 15 × 10−3 μM−1min−1). The findings herein highlight a new activity of PmADO, which may be applied as a biocatalyst for the industrial synthesis of fatty alcohols. [Display omitted] •PmADO was found to have the ability to generate fatty alcohols from fatty aldehydes under anaerobic conditions.•This activity of PmADO requires only NAD(P)H and does not involve metal cofactors.•PmADO has a preference for medium-chain aldehydes, specifically octanal.•The new activity of PmADO may have potential for use as a biocatalyst in industrial synthesis of fatty alcohols.