The mutagenic activity of ethylmethanesulphonate, benzidine and benzo[a]pyrene at the hprt locus of wild-type L5178Y mouse lymphoma cells

• Published in: Mutagenesis Vol. 5 Suppl; p. 21
• Main Authors: Kennelly, J C, Clare, C B, Campbell, J, Lane, M P, Harrington, D H, Cole, H, Garner, R C
• Format: Journal Article
• Language: English
• Published: England 1990
• Subjects: Animals; Benzidines - metabolism; Benzidines - toxicity; Benzo(a)pyrene - metabolism; Benzo(a)pyrene - toxicity; Biotransformation; Cell Survival - drug effects; Ethyl Methanesulfonate - toxicity; Hypoxanthine Phosphoribosyltransferase - genetics; Leukemia L5178; Mice; Microsomes, Liver - metabolism; Mutagenicity Tests - methods; Mutagens - metabolism; Mutagens - toxicity; Mutation; Tumor Cells, Cultured
• ISSN: 0267-8357
• DOI: 10.1093/mutage/5.Supplement.21

Ethylmethanesulphonate (EMS), benzidine (BZD) and benzo[a]pyrene (B[a]P) were assayed for ability to induce mutation at the hprt locus of wild-type L5178Y mouse lymphoma cells. EMS was assayed in the absence of metabolic activation, B[a]P in the presence of metabolic activation (S-9 mix) and BZD both in the absence and presence of S-9. Treatment with EMS minus S-9 and B[a]P plus S-9, especially when the S-9 content of the incubation was 2% (v/v), produced strong dose-related increases in mutant frequency. BZD failed to induce mutation at the hprt locus, either in the absence or presence of S-9.