Optimization of Total RNA Isolation Method from the Aromatic Medicinal Plant Artemisia annua L

This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant. This innovative protocol ensures a consistently high quantity and good quality of total RNA without any contamination of poly...

Full description

Saved in:
Bibliographic Details
Published inInternational Journal of Plant Biology Vol. 3; no. 1; p. e7
Main Authors Appalasamy, Suganthi, Shu Ping, Ning, Bhatt, Arvind, Othman, Ahmad Sofiman, Jelodar, Nad-Ali Babaeian, Lai Keng, Chan
Format Journal Article
LanguageEnglish
Published MDPI AG 10.12.2012
Subjects
Artemisia annua L., tissue cultured leaves, total RNA extraction, PCR
Online AccessGet full text

Cover

More Information
Summary:This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant. This innovative protocol ensures a consistently high quantity and good quality of total RNA without any contamination of polyphenols, polysaccharides and proteins. The total RNA obtained is also free of fungal RNA even when extracted from fungal infested plants. The extraction buffer used in the proposed modified protocol was made up of non-hazardous chemicals. High concentrations of polyphenols of A. annua L. could be successfully eliminated and the prepared total RNA could be used for downstream reactions.
ISSN:2037-0164
2037-0156
2037-0164
DOI:10.4081/pb.2012.e7