Cytotoxic and genotoxic effects of [Ru(phi) 3] 2+ evaluated by Ames/ Salmonella and MTT methods

• Published in: European journal of medicinal chemistry Vol. 44; no. 6; pp. 2601 - 2605
• Main Authors: Benkli, Kadriye, Tunali, Yağmur, Cantürk, Zerrin, Artagan, Öge, Alanyali, Filiz
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Masson SAS 01.06.2009; Elsevier
• Subjects: 9,10-Phenanthrenequinone diimine; Animals; Biological and medical sciences; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Chemical mutagenesis; Cytotoxic effect; DNA Repair - drug effects; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Frameshift Mutation; Genotoxicity; Glioma - drug therapy; Glioma - pathology; Medical sciences; Molecular Structure; Mutagenicity; Mutagenicity Tests; Organometallic Compounds - chemical synthesis; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Rats; Ru(II) complex; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Tetrazolium Salts - chemistry; Thiazoles - chemistry; Toxicology; Cytotoxic effect; 9,10-Phenanthrenequinone diimine; Mutagenicity; Ru(II) complex; Genotoxicity; Cationic complex; Divalent metal Complexes; Rat; Toxicity; Cytotoxicity; Mutagen; Salmonella typhimurium; MTT assay; Bacteria; Enterobacteriaceae; Ruthenium II Complexes; Glial cell; Quinone imine; Mutagenicity testing; Rodentia; Organic ligand; Transition metal Complexes; Vertebrata; Mammalia; Cell line; Ames test; Platinoid Complexes
• ISSN: 0223-5234; 1768-3254
• DOI: 10.1016/j.ejmech.2008.09.017

In this work, we synthesized and evaluated the cytotoxic effect of [Ru(phi) 3] 2+, on rat C6 glioma cell line. Cell viability was determined by assay with 3-(4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mutagenicity of [Ru(phi) 3] 2+ was studied in vitro by using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) were used in plate incorporation assay in the absence of metabolic activation. According to the results, the Ru compound is not toxic but mutagenic, and it shows cytotoxic effect towards C6 rat glioma cells in100 μM. [Display omitted]