Study of the content of inorganic polyphosphates in Saccharomyces cerevisiae grown on different carbon sources with different O2 concentrations in the medium

• Published in: Microbiology (New York) Vol. 77; no. 5; pp. 541 - 546
• Main Authors: Vagabov, V. M., Trilisenko, L. V., Kulakovskaya, E. V., Kulaev, I. S.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.10.2008
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology; Saccharomyces cerevisiae; catabolite repression; aeration; inorganic polyphosphates; carbon source; yeast
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261708050056

The content of different fractions of inorganic polyphosphates (polyP) was studied in Saccharomyces cerevisiae VKM Y-1173 growing on a complete medium with glucose under hypoxia and active aeration as well as on ethanol. The highest growth rate was observed for aerobic fermentation, while the yield of biomass was maximal for cultivation on ethanol. In the mid-log growth phase, the amount of poly P was maximal in the cells grown on glucose under hypoxia and minimal on ethanol. In this latter case, the content of different poly P fractions changed unevenly: polyP3, polyP4, and polyP1 decreased by approximately 60%, 45%, and 30%, respectively; the salt-soluble polyP2 remained at almost the same level; while polyP5 abruptly increased 10-to 15-fold. These findings demonstrate that the metabolic pathways for poly P fractions are different. A significant drop in the amount of the main poly P fractions accompanied by a decrease of the poly P average chain length in the presence of carbon and P i sources in the medium is evidence of active involvement of poly P as additional energy sources in the flows of energy in actively growing yeast cells.