DNA methylation in tissues of Chamaedorea elegans

• Published in: Journal of forestry research Vol. 23; no. 3; pp. 425 - 428
• Main Authors: Lu, Yongquan, Qing, Jia, Li, Haiying, Tong, Zaikang
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 2012; Northeast Forestry University; The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an 311300, China
• Subjects: AFLP技术; amplified fragment length polymorphism; Biomedical and Life Sciences; Chamaedorea elegans; developmental stages; DNA methylation; DNA甲基化; Forestry; leaves; Life Sciences; loci; Original Paper; plant development; roots; 发育阶段; 多态位点; 扩增片段长度多态性; 植物生长发育; 甲基化水平; 组织分化; DNA Methylation; tissue; leaf; root; stem; Chamaedorea elegans
• ISSN: 1007-662X; 1993-0607
• DOI: 10.1007/s11676-012-0231-y

DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% （42/220） bases were uniformly methy- lated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf （L1）, young leaf （L2）, baby leaf （L3）, stem （S）, young root （R1） and lignified root （R2） were 29.5%, 29.0%, 27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of poly- morphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging.