PDGF-induces the glutathione-dependent enzyme PGH2/PGE2 isomerase in NIH3T3 and pEJ transformed fibroblasts

• Published in: Biochemical and biophysical research communications Vol. 198; no. 1; pp. 298 - 303
• Main Authors: KELNER, M. J, UGLIK, S. F
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 14.01.1994
• Subjects: 3T3 Cells; Animals; Biological and medical sciences; Cell Line, Transformed; Cell physiology; Cell Transformation, Neoplastic; Dinoprostone - analysis; Dinoprostone - metabolism; Enzyme Induction; Fibroblasts - drug effects; Fibroblasts - enzymology; Fundamental and applied biological sciences. Psychology; Genes, ras; Glutathione - pharmacology; Intramolecular Oxidoreductases; Isomerases - biosynthesis; Isomerases - metabolism; Kinetics; Mice; Microsomes - enzymology; Molecular and cellular biology; Platelet-Derived Growth Factor - pharmacology; Prostaglandin-E Synthases; Radioimmunoassay; Responses to growth factors, tumor promotors, other factors; Cell culture; Intramolecular oxidoreductases; Enzyme; Arachidonic acid derivatives; Rodentia; Prostaglandin E2; Prostaglandin-E synthase; Signal transduction; Vertebrata; Isomerases; Mammalia; Enzymatic activity; Mouse; Polypeptide; Fibroblast; Platelet derived growth factor; Growth factor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1994.1042

Exposure of NIH3T3 and pEJ serum-starved cells to platelet derived growth factor results in a 16 fold increase in the glutathione-dependent enzyme prostaglandin H2/prostaglandin E2 isomerase activity (EC 5.3.99.3). The response is rapid as a detectable increase in NIH3T3 cells occurs after only 7 minutes of exposure to the growth factor. Only a mild increase in another microsomal glutathione-dependent enzyme, microsomal glutathione transferase (EC 2.5.1.18), was detected after a 2 hour exposure to the growth factor.