G12 and G13 α-subunits are immunochemically detectable in most membranes of various mammalian cells and tissues

• Published in: Biochemical and biophysical research communications Vol. 198; no. 3; pp. 906 - 914
• Main Authors: SPICHER, K, KALKBRENNER, F, ZOBEL, A, HARHAMMER, R, NÜRNBERG, B, SÖLING, A, SCHULTZ, G
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 15.02.1994
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Antibodies; Antibody Specificity; Binding and carrier proteins; Biological and medical sciences; Cell Membrane - metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; GTP-Binding Proteins - analysis; GTP-Binding Proteins - biosynthesis; Humans; Immunoblotting; Macromolecular Substances; Mammals; Molecular Sequence Data; Neurons - metabolism; Oligopeptides - chemical synthesis; Oligopeptides - immunology; Pertussis Toxin; Proteins; Rabbits - immunology; Signal Transduction; Tumor Cells, Cultured; Virulence Factors, Bordetella - pharmacology; Binding protein; Tissue; GTP; Vertebrata; Molecular form; Mammalia; Alpha-Peptide chain; Gene expression; Brain (vertebrata); G protein
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1994.1129

The cDNAs of two putatively pertussis toxin-insensitive G-protein alpha-subunits, alpha 12 and alpha 13, were recently cloned. mRNA analyses based on the reverse transcriptase polymerase chain reaction indicated a widespread distribution of both mRNAs [Strathmann, M. P., and Simon, M. I. (1991) Proc. Natl. Acad. Sci. USA 88, 5582-5586]. Generating specific antibodies directed against internal and C-terminal peptide sequences, we identified alpha 12 protein in all and alpha 13 protein in most tissues and cell lines tested. No species differences were observed, indicating a high degree of identity between mammalian species. Strong immunoreactive signals of both proteins were obtained in neuronal cell membranes of various species. Our results support the hypothesis that G12 and G13 are involved in pertussis toxin-insensitive pathways of signal transduction common to most tissues.