Nature of the Conversion of Ficus carica Variety Kadota Ficin Component D to Component C. Some Physicochemical Properties of Components C and D

Component C can be formed from component D under the experimental conditions used during purification of Ficus carica variety Kadota latex. By use of the inhibitor, sodium p-chloromercuribenzoate, the 2 components have been purified to chromatographic homogeneity. The 2 components have identical mol...

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Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 44; no. 11; pp. 1566 - 1573
Main Authors Donald E. Kramer, Whitaker, John R.
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.11.1969
Subjects
Amides
Amino acids
Chemical bonding
Chromatography
Disulfides
Enzymes
Gels
Latex
Molecular weight
Sodium
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Summary:Component C can be formed from component D under the experimental conditions used during purification of Ficus carica variety Kadota latex. By use of the inhibitor, sodium p-chloromercuribenzoate, the 2 components have been purified to chromatographic homogeneity. The 2 components have identical molecular weights and amino acid composition. The only difference found between the 2 components is the presence of 3 to 6 more amide groups in component D than in component C. There also appears to be a conformational difference between the 2 since component C is not as acidic, with respect to component D, as would be expected from the comparative amide contents. Conformational differences between the 2 are also indicated by the chromatographic behavior of the 2 in the presence and absence of sodium-p-chloromercuribenzoate.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.44.11.1566