Seasonal dynamic changes in bacterial compositions in the Inner Mongolia desert steppe

• Published in: Frontiers of biology in China Vol. 4; no. 2; pp. 187 - 191
• Main Authors: Lu, Ping, Ma, Wanli, Li, Hao, Wu, Yongsheng, Lv, Guifen, He, Jizheng
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Higher Education Press 01.06.2009
• Subjects: Biochemistry; Biomedical and Life Sciences; Cell Biology; Developmental Biology; Genetics and Population Dynamics; Life Sciences; Neurobiology; Proteobacteria; Proteomics; Research Article; Inner Mongolia desert steppe; community composition; seasonal dynamic change; Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)
• ISSN: 1673-3509; 1673-3622
• DOI: 10.1007/s11515-009-0002-0

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was employed to examine the seasonal dynamic changes in bacterial community composition in the Inner Mongolia desert steppe using specific primers F954 and R1369. Bright and reproducible bands were sequenced, and the phylogenic tree was constructed. The results show that the bacterial community composition changed between different seasons. The specific bands were different between the sampling sites with light and heavy levels of degraded grassland. Three main types of bacteria constituting the microbial community in the Inner Mongolia desert steppe belonged to the α, γ andgd-sub phyla of Proteobacteria, Bacteroidetes and Acidobacteria. The unculturable bacteria accounted for 69% of the whole bacterial community of the Inner Mongolia desert steppe.