Nanoformulating Cinnamomum zeylanicum essential oil with an extreme effect on Leishmania tropica and Leishmania major

• Published in: Journal of drug delivery science and technology Vol. 63; p. 102436
• Main Authors: Ghanbariasad, Ali, Valizadeh, Alireza, Ghadimi, Shamsi Noorpisheh, Fereidouni, Zhila, Osanloo, Mahmoud
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2021
• Subjects: Cinnamomum zeylanicum; Cutaneous leishmaniasis; Leishmania major; Leishmania tropica; Nanoemulsion; Cutaneous leishmaniasis; Leishmania tropica; Cinnamomum zeylanicum; Nanoemulsion; Leishmania major
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2021.102436

Major causatives for cutaneous leishmaniasis, the most common form of leishmaniasis, in the old world are Leishmania major and Leishmania tropica. Investigating the leishmanicidal effects of essential oils and preparing their nano/formulation is a promising approach for developing new green drugs. In this study, the ingredients of Cinnamomum zeylanicum essential oil were investigated using GC/MS analysis, and its antioxidant effect was then evaluated using the DPPH assay. Moreover, the leishmanicidal effect of the essential oil against L. major and L. tropica was investigated; obtained half-maximal inhibitory concentrations (IC50)s were 16.53 and 7.56 μg/mL. After that, a practical dosage form of C. zeylanicum, i.e., the nanoemulsion with a 52 ± 4 nm droplet size and a low droplet size distribution index (SPAN = 0.97), was prepared. Interestingly, after treatment of both species with nanoemulsion, their viability was reduced to 0%. The prepared nanoformulation could be considered a potent anti-leishmanial agent for other species and in vivo studies. [Display omitted]